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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in several tumors, which leads to chromosome instability, and therefore may perhaps contribute to tumorigenesis. This protein was observed to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in endorsing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are explained. Two more splice variants were described but thorough nucleotide sequence data is not really nonetheless obtainable.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This guidebook outlines further feasible optimization of cellular and immunoassay parameters to be sure the very best effects are...
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This information outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope over the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, you can see which staining is unique.
Focus on Details Cyclin E belongs to your hugely conserved cyclin household, whose customers are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show unique expression and degradation styles which lead for the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates in the G1-S phase boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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